David Tennant and Olivia Colman who played DI Alec Hardy and DS Ellie Miller in the ITV Show will take part in the event at Winchester Cathedral on December 7.
It is in aid of Breast Cancer Haven in Titchfield and The Murray Parish Trust.
Other high profile personalities from the world of TV and film who will take part include Downton Abbey star Hugh Bonneville and actor Timothy West CBE.
There will also be a performance by renowned soprano Claire Rutter, along with festive performances by a variety of Hampshire musical groups including Winchester Cathedral Girl Choristers.
BBC South Today presenter and chair of The Breast Cancer Haven Development Board Sally Taylor said:“Once again, we are extremely grateful for this generous support from our high profile friends. We are delighted to be working closely with The Murray Parish Trust to deliver something very special for Winchester Cathedral this year.”
Actress and co-founder of The Murray Parish Trust, Sarah Parish said: “We are so lucky to have such big names supporting this event and we will be doing everything we can to ensure that this will be a night to remember to raise as much funds as possible for these two incredible local charities.”
From September 600 tickets will be available. To register your interest or if you would like to be involved with or support the event in any way, contact Sally Hillyear on Sally.Hillyear@breastcancerhaven.org.uk or Sam Robson on firstname.lastname@example.org